BEACON Team

Dr David Bryant

BEACON Co-Investigator

dgb@aber.ac.uk

Aberystwyth University

Background

I specialise in applied biochemistry and molecular biology with the main emphasis of my research focussing on the enzymatic release and bioconversion of plant cell-wall carbohydrates to bio-renewable platform chemicals such as ethanol and/or lactic acid. Having returned to academia, I am now deploying the knowledge and experience gained in the biopharmaceutical industry to developing novel approaches for biorefining and bioconversion of renewable, sustainable feed stocks in collaboration with small to medium sized enterprises. My PhD research at Durham University was funded by the BBSRC and Bayer Crop Science, Lyon focussed on understanding pesticide detoxification in food crops, weeds and plant fungal pathogens.

Research Interests

  • Biorefining and bioconversion processes.
  • Cloning and expression of recombinant proteins and their application in a biorefining tool kit.
  • Enzymology of plant cell-wall deconstruction.
  • Pretreatment technologies to enhance the enzymatic hydrolysis of lignocellulosic biomass.
  • Application of dielectric capacitance probes to monitor carbohydrate release during simultaneous saccharification and fermentation of lignocellulose.
  • Modelling saccharification and fermentation parameters for the production of bio-renewable fuels and platform chemicals.
  • Metabolic engineering of bacteria for the production of bio-renewable fuels and chemicals.
  • Optimisation of bioconversion using Design of Experiments (DoE) methodologies.

PhD Studentships

Mr Alun T. Hughes researching - "Improvements to plant bio-refining through metabolic engineering"

New PhD studentship in enzyme design - "Improving thermostability and catalytic activity of lipases through computation modelling" - this studentship has now been appointed and is no longer available.

Selected Publications

  • Bryant DN, Morris SM, Harding G, Fish S, Donnison IS, Gallagher JA (2012) An on-farm, pilot scale process for biorefining Lolium perenne (var. Aber Magic) to bio-ethanol and multiple commodity value streams. J. Vis Exp. (accepted for publication)
  • David N. Bryant, Elliot Firth, Naheed Kaderbhai, Stephen Taylor, Stephen M Morris, Daniel Logan, Naroa Garcia Andrew Ellis, Steven M. Martin, Joe A Gallagher (2012) Monitoring real-time enzymatic hydrolysis of Distillers Dried Grains with Solubles (DDGS) by dielectric spectroscopy following hydrothermal pre-treatment by steam explosion. Accepted in press, Bioresource Technology, August 2012. 10.1016/j.biortech.2012.09.021
  • Bryant David N, Stephen M Morris, David Leemans, Stephen Taylor, John Carvel, Robert W. Todd, Daniel Logan, Matthew Lee, Naroa Garcia, Andrew Ellis and Joe A Gallagher. (2011) Modelling real-time simultaneous saccharification and fermentation of lignocellulose and monitoring organic acid accumulation on-line using dielectric spectroscopy. Bioresource Technology. 102. 9675-9682.
  • Farrar K, Bryant D, Turner L, Gallagher J, Thomas A, Farrell M, Allison G, Humphreys MO and Donnison IS. (2011) Breeding for Bio-ethanol Production in Lolium perenne L.: Association of Allelic Variation with High Water-Soluble Carbohydrate Content. October.
  • Cummins Ian, Bryant David N and Edwards Robert (2009). Safener responsiveness and multiple herbicide resistance in the weed black-grass (Alopecurus myosuroides). Plant Biotechnology. 7(8):807-20.

Patents

  • David N. Bryant, S. M. Morris, Graham Harding, Steven Fish, Iain S. Donnison, Joe A. Gallagher et al. (2012) Biofuel from Grass. Patent Application Number 1214338.4. Filing Date: 10 August 2012.

Conferences and abstracts

  • David N Bryant, Stephen M Morris, David Leemans, Stephen Taylor, John Carvel, Robert W. Todd, Daniel Logan, Matthew Lee, Naroa Garcia, Andrew Ellis and Joe A Gallagher. (2011) Enzymatic hydrolysis of lignocellulose; the challenge of determining feedstock depletion and biomass growth on-line during simultaneous saccharification and fermentation. Invited speaker at Biofuels in the Uk: Challenges and opportunities. The Institute, East Finchley, London, N2 8LL, United Kingdom.
  • David N Bryant, Stephen M Morris, David Leemans, Stephen Taylor, Steve Fish, John Carvel, Robert W. Todd, Daniel Logan, Matthew Lee, Naroa Garcia, Andrew Ellis and Joe A Gallagher (2011) Application of dielectric annular spectroscopy for on-line monitoring of enzymatic hydrolysis of perennial rye-grass fibre. Speaker at the 33rd Symposium on Biotechnology for Fuels and Chemicals. A specialized conference of the Society for Industrial Microbiology. Sheraton Hotel, Seattle, Washington.
  • Monitoring fibre breakdown in real time by capacitance – application to platform chemical and biofuel production. David N Bryant, Stephen M Morris, David Leemans, Stephen Taylor, Steve Fish, John Carvel, Robert W. Todd, Daniel Logan, Matthew Lee, Naroa Garcia, Andrew Ellis and Joe A Gallagher (2011) Speaker at the Low Carbon Research Institute (LCRI) conference, National Museum of Wales & Welsh School of Architecture, Cardiff.
  • Joe A. Gallagher, Lesley B Turner, Kerrie Farrar, Leif Skot, David N. Bryant, Emma Timms-Taravella, Mike S Morris, Markku Farrell, Ann Thomas and Iain S Donnison (2011) Improving water soluble sugar content in perennial rye-grass: Developing grasses for production of biofuels and bulk chemicals. 33rd Symposium on Biotechnology for Fuels and Chemicals. A specialized conference of the Society for Industrial Microbiology. Sheraton Hotel, Seattle, Washington.
  • Stephen M. Morris, David N. Bryant, Dave S Thomas, Steve Fish and Joe A. Gallagher (2011) Grassohol - Conversion of high sugar grasses to alcohol based transport fuel. 33rd Symposium on Biotechnology for Fuels and Chemicals. A specialized conference of the Society for Industrial Microbiology. Sheraton Hotel, Seattle, Washington.